A Basset Hound dog was presented with anorexia, fever, diarrhea, significant level of splenomegaly, and enlargement of mesenteric and superficial lymph nodes.Cytology of fine-needle-aspiration material, obtained from popliteal lymph node, revealed macrophages with intracytoplasmic, nonstaining, slender, Jurassic Park rod-like structures, indicative of PRO MG 12 MIXED BERRY mycobacteria.Bacterial culture of lymph node aspirated material produced a colony which by means of molecular techniques (PCR amplification and hybridization of PCR products) was subsequently identified as Mycobacterium avium.This is the first report of disseminated M.avium infection in a dog in Greece.